Code | Completion | Credits | Range | Language |
---|---|---|---|---|
17ABBLT | Z,ZK | 4 | 2P+2L | English |
The course 17ABBLT can be graded only after the course 17ABBFCH has been successfully completed.
The course 17ABBLT can be graded only after the course 17ABBBCH has been successfully completed.
Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.
During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.
Credit requirements:
Mandatory attendance at all tutorials. Students have to come in time and be
well prepared. Absences are acceptable for serious reasons only, have to be
apologized and recompensed.
At every entrance-test, students have to score at least 60% of total points
to be admitted to the tutorial; if not, the tutorial (including the test)
will be recompensed on another date. A maximum of one tutorials can be
recompensed in total.
Working out all assigned exercises and submitting laboratory reports in
satisfying quality.
Successfully passing the credit-test.
Final examination:
Students have to pass a written test comprising the topics discussed during
both lectures and tutorials. The exam is graded according to the ECTS
scale.
1. Princles of bioanalytical methods and clinical diagnostic
2. Optical methods I
3. Optical methods II
4. Electrochemical methods
5. Electrochemical methods for measuring blood gases
6. Biosensors and automation
7. Electromigration methods
8. Genetic techniques
9. Imunochemical methods
10. Membrane processes
11. Centrifugation and extraction
12. Chromatography I
13. Chromatography II
14. Mass spectrometry
1. Laboratory safety rules
2. UV-VIS spectrophotometry
3. Potenciometric titration of aminoacids
4. Agar elfo
5. Measurement of concentration/partial pressure of CO2 with gas probe, Measurement of partial pressure of O2 with galvanic probe
6. Gel chromatography
7. Simulation fo HIV detection by ELISA
Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.
1. Kenkel, John. Analytical chemistry for technicians. 4th ed. Boca Raton: CRC Press/Taylor & Francis, 2014. ISBN 978-1-4398-8105-7
2. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.
3. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.
4. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.
5. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.
6. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.
7. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.
Attachment | Size |
---|---|
1. Principle of methods in clinical laboratory | 1.07 MB |
2. Optical methods I. | 3.4 MB |
3. Optical methods II. | 2.59 MB |
4. Electrochemical methods | 627.72 KB |
5. Biosensors and laboratory automation | 4.28 MB |
6. Electromigration methods | 6.04 MB |
7. Genetic methods | 3.06 MB |
8. Immunochemical methods | 839.81 KB |
9.,10. Chromatography | 908.21 KB |
11. Mass spectrometry | 941.65 KB |
12. Genetic methods II | 2.83 MB |
Attachment | Size |
---|---|
1. Laboratory safety rules | 146.84 KB |
1. Preanalytics HW | 142.63 KB |
2. Preparation of solution and basic pipetting I., II. | 635.41 KB |
4. Optical methods | 88.26 KB |
4. Optical methods HW | 413.67 KB |
5. UV-VIS spectrophotometry | 325.92 KB |
6. Measurement of concentration/partial pressure of CO2 with gas probe | 744 KB |
7. Measurement of partial pressure of O2 with galvanic probe | 310.08 KB |
8. Agarose gel electrophoresis | 207.43 KB |
8. Electromigration method HW | 94.38 KB |
9. Potenciometric titration of aminoacids | 410.42 KB |
10. Simulation of HIV detection by ELISA | 470.22 KB |
11. Dialysis | 174.46 KB |
12. TLC of aminoacids | 243.55 KB |
13. Gel chromatography | 242.46 KB |
Attachment | Size |
---|---|
Model exam test | 491.9 KB |
Schedule of lectures: https://harm.fbmi.cvut.cz/B222/17ABBLT/lec
Schedule of tutorials: https://harm.fbmi.cvut.cz/B222/17ABBLT/lab