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F7ADIBKDS - Methods of Work with a Cell Culture and Dynamic Systems

Code Completion Credits Range Language
F7ADIBKDS ZK 20P+8C English
Lecturer:
Lucie Bačáková (guarantor)
Tutor:
Roman Matějka, Jana Štěpanovská
Supervisor:
Department of Biomedical Technology
Synopsis:

Methods and procedures in tissue engineering, work with cell culture, preparation of carriers, testing of biocompatibility and cytotoxicity, use of dynamic systems for cell culture.

Requirements:

As a standard, teaching takes place in contact form and the course ends with an oral exam, which is preceded by written preparation. If the number of students is less than 5, teaching can take place in the form of guided self-study with regular consultations. Furthermore, it is required to prepare a written study by the student on a given topic in the field. The condition for admission to the exam is the completion of two laboratory exercises (evidenced by a protocol signed by the student, the head of the exercise and the guarantor of the course). Protocols will be archived.

Syllabus of lectures:

Syllabus of lectures:

1. In-vitro cell culture procedures

2. Cell cultures, lines, primary cultures

3. Stem cells

4. Methods of tissue acquisition, cell separation

5. Artificial and biological cell carriers,

6. Methods of imaging cell cultures

7. Biocompatibility of tissue carriers covering them to reduce cytotoxicity and increase proliferation

8. Cell stimulation (mechanical, chemical, electrical) supporting proliferation, gene expression and differentiation

9. Perfusion systems providing conditions for cell growth and stimulation

10. Tissue decellularization, crosslinking and carrier crosslinking

Syllabus of tutorials:

Syllabus of exercises (block form of teaching after 4 teaching hours):

1. Basics of working with cells, ie working in a laminar box, ensuring sterility and minimizing contamination, passage of cells, deployment on a cell carrier and preparation for dynamic cultivation, flow systems of the PPFC type,

2. Systems for mechanical loading of cell culture, preparation of gel substrates, mechanical loading in a bioreactor

Study Objective:

Methods and procedures in tissue engineering, work with cell culture, preparation of carriers, testing of biocompatibility and cytotoxicity, use of dynamic systems for cell culture.

Study materials:

[1] Lanza, R., Langer, R., Vacanti, J., Principles of Tissue Engineering, ed. 3rd Edition , Elsevier Academic Press, 2007, ISBN 978-0123706157

Note:
The course is a part of the following study plans:
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