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F7ABBLT - Clinical Laboratory Instrumentation

Code Completion Credits Range Language
F7ABBLT Z,ZK 4 2P+2L English
Grading of the course requires grading of the following courses:
Biochemistry (F7ABBBCH)
Physical Chemistry (F7ABBFCH)
Martina Turchichová (guarantor), Stanislav Gajdoš
Lenka Strnadová, Martina Turchichová (guarantor), Kateřina Dunovská, Stanislav Gajdoš
Department of Biomedical Technology

Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.

During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.


Assessment requirements:

Active attendance at all tutorials. Students has to come in time and be well prepared. Absences are acceptable for serious reasons only, have to be apologized and recompensed. Passing entrance test at least for 50% for each tutorial; if not, the tutorial including the test will be recompensed on another date.

Working out assigned exercises and submiting laboratory reports in satisfying quality.

Examination conditions: Student has to pass a written test comprising the topics discussed during the lectures and tutorials, including assigned individual study. The exam is graded according to the ECTS scale, to pass the exam, the test must be evaluated at least at 50 %.

Syllabus of lectures:

1. Princles of bioanalytical methods and clinical diagnostic

2. Optical methods I

3. Optical methods II

4. Electrochemical methods (galvanic sond for dissolved oxygen measurement, alternatives for Clark probe, advantages and disadvantages of its use, methods of calibration)

5. Electrochemical methods for measuring blood gases (Astrup, instrumentation of methodd, used electrodes - its type and construction, calculation of potentials of electrodes, gas CO2 probe - specifics of measurement, factors affectig measurement, right experimantal conditions setup)

6. Biosensors and automation

7. Electromigration methods

8. Genetic techniques

9. Imunochemical methods

10. Membrane processes

11. Centrifugation and extraction

12. Chromatography I

13. Chromatography II

14. Massí spectrometry

Syllabus of tutorials:

1. Laboratory safety rules

2. Buffers preparation, principles of statistical evaluation of experimental results

3. Potenciometric titration of aminoacids.

4. Agarose gel electrophoresis

5. Electrochemical techniques of measurement dissolved gases (Measurement of concentration/partial pressure of CO2 with gas probe, Measurement of partial pressure of O2 with galvanic probe)

6. Gel chromatography

7. Simulation of HIV detection by ELISA

Study Objective:

Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.

Study materials:

1. Kenkel, John. Analytical chemistry for technicians. 4th ed. Boca Raton: CRC Press/Taylor & Francis, 2014. ISBN 978-1-4398-8105-7

2. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.

3. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.

4. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.

5. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.

6. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.

7. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.

The course is a part of the following study plans: