F7ABBBCH - Biochemistry

Students will be introduced to fundamental areas of biochemistry and will expand their knowledge of the chemistry of living systems. The course covers the basic building blosks of biological systems (amino acids, proteins, lipids, carbohydrates, nucleic acids), biological membranes and the transmission of biological signals, to the most important metabolic processes and diseases.

Particular attention is paid to the aspects necessary for understanding working methods in biochemical and clinical laboratories, which form part of the skill set required of a biomedical technician. The laboratory sessions focus on the practical application of the theoretical knowledge acquired. Students will master the basic laboratory techniques of biochemistry.

Credit requirements:

Compulsory attendance at all laboratory sessions. Before each laboratory session the introductory test must be completed (minimum 50 % score). Any absences must be properly excused and made up. A maximum of two lab exercises may be substituted. Submission of completed reports in sufficient quality by the end of the 14th week of semester.

Examination procedure:

The examination consists of a written test. The scope of the test covers all topics discussed in the lectures and laboratory sessions. The weighting of questions varies according to their difficulty. To pass the exam, a minimum of 50 per cent must be obtained. Test is evaluated according to the ECTS scale.

1. Biochemical principles

3. Biomolecules - composition of human organism

5. Protein, their composition and function

7. Biomembranes and transfer of information

9. Principles of metabolism and transfer of energy

11. Nutrition and metabolic disorders

13. Homeostasis and integrity of organism

The basis of the exercise:

1. Laboratory regulations, safety training and introduction to biochemistry laboratory work

1. Preparation of solutions and basics of pipetting

3. TLC of amino acids and DNA isolation

5. Properties, precipitation and denaturation of proteins

7. Properties and determination of carbohydrates

9. Properties of soaps and determination of enzyme activity

11. Compensation of exercises and completing the credit requirements

Students will gain an understanding of the fundamental areas of biochemistry and the interrelationships between them. They will be able to understand biochemical concepts, particularly in the context of clinical biochemistry. In the laboratory, they will learn to work in accordance with good laboratory practice, become familiar with the specifics of working with biological material, and develop good working habits. They will be able to correctly process, interpret and discuss the results obtained.

[1] LIEBERMAN, Michael a Rick E. RICER. Biochemistry, molecular biology, and genetics. 6th ed. Philadelphia, 2014.

[2] Murray, R.K., et al.: Harper´s illustrated biochemistry. Mc Graw Hill Medical, New York 2009.

[3] Nelson, D.L., Cox, M.M.: Lehninger principles of biochemistry, WH Freeman, New York 2008.

[4] Voet, D., Voet, J.G.: Biochemistry. Wiley, Hoboken 2011.

• Prospectus - bakalářský (!)
• Biomedical Technology (compulsory course)
• Biomedical Technology (compulsory course)