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17AMBLT - Clinical Laboratory Instrumentation

Code Completion Credits Range Language
17AMBLT Z,ZK 4 1+1
Lenka Strnadová (guarantor)
Lenka Strnadová (guarantor)
Department of Biomedical Technology

Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.

During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.


Credit requirements:

Active attendance at all tutorials. Students has to come in time and be well prepared. Absences are acceptable for serious reasons only, have to be apologized and recompensed. Passing entrance test at least for 50% for each tutorial; if not, the tutorial including the test will be recompensed on another date.

Working out assigned exercises and submiting laboratory reports in satisfying quality.

Examination conditions: Student has to pass a written test comprising the topics discussed during the lectures and tutorials, including assigned individual study. The exam is graded according to the ECTS scale, to pass the exam, the test must be evaluated at least at 50 %.

Syllabus of lectures:

1. Principles of method in clinical laboratory

2. Optical methods I

3. Optical methods II.

4. Elektrochemical methods I

5. Elektrochemical mthods II, Biosensors, automation in clinical laboratory

6. Elektromigration methods

7. Genetic methods

8. Imunochemical methods

9. Membrane processes

10. Chromatography I

11. Chromatography II

12. Lecture cancelled

13. Extraction and centrifugation.

14. Mass spectrometry I+II

Syllabus of tutorials:

1. Laboratory safety rules

2. Lab: Buffer preparation. Basics of pipetting.

3. Theory: Principles of bioanalytical methods in a clinical laboratory

4. Theory: UV-VIS spectrometry, turbidimetry and nephelometry I

5. Theory: UV-VIS spectrometry, turbidimetry and nephelometry II

6. Lab: Spectrophotometric evaluation of proteins

7. Lab: Titration curves of aminoacids

8. Theory: Agarose electrophoresis I

9. Lab: Agarose electrophoresis II + evaluation of resuls

10. Theory: principle of ELISA methods. Chromatography

11. Lecture cancelled

12. Lab: Thin layer chromatography

13. Lab: Mass spectrometry construction + inspection of machine.

14. Revision of topics. Compensation of laboratories.

Study Objective:

Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.

Study materials:

1. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.

2. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.

3. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.

4. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.

5. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.

6. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.

The course is a part of the following study plans: