17ABBBCH - Biochemistry

Course participants will be introduced to the basics of Biochemistry. The course builds on the knowledge gained in general chemistry and extends this knowledge about the chemistry of living systems. The interpretation goes through the basic building structures of biological systems (amino acids, peptides, proteins, lipids, carbohydrates, nucleic acids), biological membranes and molecular genetics to the most important metabolic processes. Particular attention is paid to the aspects necessary for understanding the methods of work in the biochemical and clinical laboratory, which are part of the follow-up chemical discipline.

The laboratories are focused on broadening the topics discussed in the lectures and their practical training, especially on the determination of biomolecules and the verification of their properties. Students should become familiar with the basic laboratory techniques of Biochemistry.

Conditions of classified assesment:

Obligatory and active attendance at all tutorials. Absences are acceptable for serious reasons only and must be apologized and recompensed.

For the attendance at each tutorial, student has to pass an entrance test at least for 50 %. In the other way, he has to recompense the session with the test on an other date. Student can recompense maximally two tutorials. Student can gain 25 points from the entrance tests, which will be part of the grading.

Student has to write and submit protocols from all lab sessions in sufficient quality.

At the end of the semester student has to pass a written final credit test. Student has one attempt for writing the final credit test. The final grade is created by the sum of both the points from the entrance tests and from the final test and in evaluated according to the ECTS scale.

1. Introduction to biochemistry

2. Nucleic acids

3. Amino-acids, peptides and proteins

4. Carbohydrates

5. Lipids and biomembranes

6. Enzymes

7. Principles of metabolism and important biochemical pathways

1. Cell, organic molecules, pH, non-covalent interactions.

2. Properties of amino acids, peptide bond, titration of amino acids and TLC.

3. Structure of saccharides and their clinical estimation.

4. Properties of lipids, lipid particles.

5. Enzyme classes, enzyme kinetics.

6. Glycolysis, citrate cycle, respiration chain.

7. Review of topics. Model test.

The goal of study is to understand the significance of biochemical compounds in living organisms, and to deduce their properties by the knowledge of their structure. After passing the course, student should be well versed in biochemical problems either of clinical diagnostics either of daily life. Further aim of this subject is to get work with scientific publications, search for biochemical information and to present obtained knowledge to other students.

[1] LIEBERMAN, Michael a Rick E. RICER. Biochemistry, molecular biology, and genetics. 6th ed. Philadelphia, 2014.

[2] Murray, R.K., et al.: Harper´s illustrated biochemistry. Mc Graw Hill Medical, New York 2009.

[3] Nelson, D.L., Cox, M.M.: Lehninger principles of biochemistry, WH Freeman, New York 2008.

[4] Voet, D., Voet, J.G.: Biochemistry. Wiley, Hoboken 2011.

• Biomedical Technician - full time study in English (compulsory course)